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PatchJet - Overview
works of  Carl C Pisaturo
A Tool for Electrophysiology -
Pushbutton Control of Pipette Pressures

The PatchJet system (photo at right) provides easy control and monitoring of micropipette pressures for patch clamping experiments as well as micro-application of drugs. Backlighted tactile switches and tactile references allow operation by feel and in any lighting conditions.

Rationale:
Typically, pipette pressures are controlled with a hodge podge of valves, syringes, manometers, etc.

PatchJet integrates precision pressure regulators,  gauges, electronically controlled valves, lighted pushbuttons, pressure sensors, quality connectors and a pulse generator into one reliable unit.  To the physiologist, the resulting simple interface reduces errors & hassles while increasing repeatability and productivity.




Parts of PatchJet System:

Inputs (rear, not visible in photo) -
House compressed air & house vacuum via pair of 1/4" push-to-connect fittings.


Pressure Source Section (upper panel) -  
Control and monitor the 4 source pressures, 2 positive and 2 negative (suction). Buffer reservoirs are present to improve response.


Pressure Selector Modules (middle 2 panels) -
User selects pipette pressure mode with lighted pushbuttons
NEU =  "neutral", open to atmosphere
POS =  low positive, to keep pipette clear while moving
NEG =  low suction, to cling to cell and make seal
BRK =  higher suction for "break-in" pulses
PUF =  higher positive for "puffing" drug from pipette
User may monitor and record output pressure, available as analog 0-5 volt signal.


Pulse Generator (bottom panel) -
User easily sets pulse duration (25 to 999mS) and fires  pulses to any module set to BRK or PUF.  2 memories provided. Pulses may also originate from external device.


Outputs (rear, not visible in photo) -
Air lines to pipettes via 1/8" OD push-to-connect fittings;
PatchJet - User Guide